ras Gene Mutations as a Prognostic Marker in Adenocarcinoma of the Human Lung without Lymph Node Metastasis1

Adenocarcinoma of the lung obtained at surgical resection was exam ined for mutation at codons 12, 13, and 61 of the oncogenes K-ras, li ras, and Vrav, using polymerase chain reaction and oligonucleotide hybridization techniques. The mutation was detected in 18 of the 115 cases (15.7%), and 15 of 18 were at codon 12, 2 were at codon 13 of li ras, and 1 was at codon 61 of \-ras. G to T transversions were most common. The ras gene mutations were more frequent in the male patients (P —¿ 0.0048). No significant differences were found to be related to stage of the disease or tumor-nodes-metastases classification between positive and negative groups of the ras gene mutations. A history of tobacco use was not always a factor contributing to mutation. Of the completely resected group without lymph node metastasis, the 5-year survival rate in the ras-positive group was 53.3%, which was significantly poorer than the 83.6% survival rate in the ras-negative group (P < 0.05). Our findings suggest that ras gene mutations may be prognostic, especially in the early stage adenocarcinoma of the lung.